DNA transfection:
A wide variety of mammalian cells can be transfected with SAINT-MIX.
SAINT-MIX has been used to transfect several cell-types/lines, some with efficiencies up to 100%.
Click below to read about:
- Roller bottle transfection with SAINT-MIX (High yield production)
- Expression profile of transfected cells in relation with the cell doubling time
- Embryonic Stem cells transfection with SAINT-MIX
- Recent SAINT-MIX Articles
Protocols:
SAINT-MIX Protocol
SAINT-MIX Sketch
Oligonucleotides:
SAINT-MIX has been demonstrated to act as an efficient translocator
of oligo-nucleotides into eukaryotic cells. The compatibility with serum is a major advantage of this delivery system. Oligonucleotide induced cell-damage will be minimal.
SAINT-MIX product description.
The SAINT-MIX delivery system is based on a cationic pyridinium head group, showing excellent bio-compatibility.
Molecular structure of SAINT-18 (1-methyl-4-(cis-9-dioleyl)
methyl-pyridinium-chlorid):
SAINT-MIX consists of SAINT and DOPE (Dioleoyl-phosphatidylethanolamine) dissolved in H2O. The SAINT-18: DOPE ratio is 1:1.
Stability experiments showed that SAINT-MIX, stored at 4°-7°C, is stable for at least one year after opening. No loss of function appeared after repeatedly opening of the vials as long as the vials are recapped tightly after use. The antibiotic properties of SAINT-MIX ensure sterility for prolonged periods.
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