Several cell lines and primary cells have been successfully profected with the SAINT-PhD Reagent,
including CHO-K1, COS-7 , HeLaS3, U373MG, 3T3, HUVEC and Human Dermal Fibroblasts.
Click below to read more about the great benefits:
- The unique advantages of protein delivery with SAINT-PhD.
- Summary of the key benefits of protein transfection by SAINT-PhD.
- Delivery of IgG-FITC using SAINT-PhD: comparison of the standard and the alternative protocol.
- How SAINT-PhD outperforms the competition.
- Recent SAINT-PhD articles
Protocols:
SAINT-PhD Protocol
SAINT-PHD Sketch
SAINT-PhD product description:
SAINT-PhD consists of a proprietary cationic pyridinium amphiphile and a helper lipid.
Upon mixture of SAINT-PhD with the protein a particle of approximately 200nm in diameter is formed. In this particle the protein is enwrapped by at least one bilayer of lipids. Furthermore, in the complex formed only non-covalent interactions are present between SAINT-PhD and the protein. The cationic amphiphiles on the surface of the particle have high affinity for the negatively charged cell surface. Upon fusion or entrapment of the particle the protein is released into the cytoplasm of the cell. The proteins delivered by SAINT-PhD are functional and unmodified.
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