Several cell lines and primary cells have been successfully profected with the SAINT-PhD Reagent,
including CHO-K1, COS-7 , HeLaS3, U373MG, 3T3, HUVEC and Human Dermal Fibroblasts.
- The unique advantages of protein delivery with SAINT-PhD.
Intracellular delivery of proteins by SAINT-PhD is not significantly affected by the
presence of serum. This is a major advantage, because most competitors (e.g. Bioporter)
are not able to deliver proteins into cells in the presence of serum. Furthermore, the presence of serum is self-evident in in vivo protein delivery experiments and applications.
- Summary of the key benefits of protein transfection by SAINT-PhD.
After delivery, the protein has not lost its activity. For example, ß-galactosidase retains
it enzymatic activity after uptake. This uptake of ß-galactosidase by CHO-K1 Cells. 1µg
was complexed with 20µL of SAINT-PhD. The complex was delivered in a 24-well plate, incubated for 4 hours and stained with X-gal.
No effect by serum.
The presence of serum in the cell culture medium does not affect the rate or level of
uptake of protein by SAINT.
- Delivery of IgG-FITC: comparison of the standard and alternative protocol.
Intracellular delivery of FITC-labeled IgG with SAINT-PhD: A range of FITC-labeled IgG
was mixed with 10µL of SAINT-PhD reagent in a total volume of 25µL. The complexes were
filled up to 125µL with total growth medium and added to the cells in a 48-well plate.
The complexes were incubated for 4 hours (37°C,5% CO2) without changing the medium. FACS analysis was performed
after harvesting the cells.
- How SAINT-PhD outperforms the competition.
SAINT-PhD protein delivery reagent has been tested together with 5 competing protein delivery reagents. Cells were plated at a density of approximately 100 thousand cells/well in a 24-well plate one day before protein transfection. It is clear that SAINT-PhD is the only reagent that performs well when
delivering relatively small amounts of proteins into cells growing in serum containing medium.
It is our opinion that these two advantages are very significant for our customers.
- Recent SAINT-PhD articles
Protocols:
SAINT-PhD Protocol
SAINT-PHD Sketch
SAINT-PhD product description:
SAINT-PhD consists of a proprietary cationic pyridinium amphiphile and a helper lipid.
Upon mixture of SAINT-PhD with the protein a particle of approximately 200nm in diameter is formed. In this particle the protein is enwrapped by at least one bilayer of lipids. Furthermore, in the complex formed only non-covalent interactions are present between SAINT-PhD and the protein. The cationic amphiphiles on the surface of the particle have high affinity for the negatively charged cell surface. Upon fusion or entrapment of the particle the protein is released into the cytoplasm of the cell. The proteins delivered by SAINT-PhD are functional and unmodified.
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