- Inhibition of EpCAM expression on SKOV-3 tumour cells.

We compared several siRNA transfection reagents by evaluating the expression inhibition of the epithelial-glycoprotein EpCAM on SKOV-3 tumor cells. The results clearly show that SAINT-RED mediated delivery of siRNA generates the highest inhibition by far compared to the competitors using only 3 pmol of siRNA.

  - High throughput siRNA screening. ( Max Planck Institute )

Synvolux Therapeutics siRNA transfection reagent SAINT-RED permits the rapid and efficient delivery of siRNA into a variety of mammalian cell lines. Here, the application of SAINT-RED in siRNA high-throughput screening is demonstrated. It also has been shown that both the forward and reverse transfection protocol yield similar results.

  - Inhibition of enzyme activity with different amounts of siRNA.

150,000 3T3 cells were seeded in a 6-well plate the day before transfection. Enzyme activity of a housekeeping gen was determined 48 hr after a single transfection of different amounts of siRNA. When 1 ug of siRNA (70 pmol) was complexed with 20 ul SAINT-RED an inhibition of 75% was found. Final concentration of siRNA is 25nM.

  - Long term preservation and high transfection efficacy of siRNA.

Long term preservation and high transfection efficacy of siRNA is achieved by use of SAINT-RED. Reproducibility and the introduction of an (internal) standard is difficult when using siRNA to knock-down gene-expression. If the experiment does have a time-spanning period (several transfections in days/months), and contains different subsets of data to be generated, it is even more complex. The siRNA could become inactive even if you aliquot the solution and store at -20°C.

  - Recent SAINT-RED Articles

 
Protocols:
SAINT-RED Protocol
SAINT-RED Sketch

 
SAINT-RED product description and protocols:

The SAINT-RED delivery system is based on a cationic pyridinium head group, showing excellent bio-compatibility.