Transformation of E.Coli is still essential in retrieving the recombinant Plasmid DNA clone of choice.
Since the introduction ( Cohen 1972) of the CaCl2 method the transformation efficacy increased
enormously and was improved in 1983 by Hanahan. With the introduction of prepacked competent cells
another significant improvement was made.
We at Synvolux developed a TRAnsformation ENhancer for E. coli strains.
The use of TRAEN will increase the amount of colonies retrieved. Depending on the plasmid length
( 3,5 ~ 10 Kb ) and purification state of the plasmid the transformation efficacy will increase by
200% and more. In a typical experiment with a 6 Kb plasmid we retrieved 5 times more colonies
using TRAEN in the transformation of commercially available competent cells. Instead of using prepacked
competent cells with TRAEN, the same results were obtained when we used cells prepared with the
CaCl2 method in combination with TRAEN.
The application of this new product is very simple.
Just add 3 ul of TRAEN to the DNA (100ng) you want to transfer to E.Coli. The rest of your protocol,
which you worked hard to optimize , can be used as it is.
Protocols:
TRAEN Protocol
|
