In general the following parameters affect transfection results:
- Purity and concentration of DNA, RNA, oligo’s or proteins
- Vector sequence (promoter, poly(A)-tail, codon usage, etc.)
- Amount of SAINT/DNA complex added
- Target cell (cell type, cell cycle, passage number, etc.)
- Protein expression (time-dependent expression levels, toxicity of expressed protein, etc.)
Like most other transfection agents and delivery systems, the best results are obtained with highly purified DNA, RNA, or proteins/peptides.
The ‘transfectability’ of cells is highly variable. For example, non-dividing and non-adherent cells are generally difficult to transfect, while other cell types may be transfected readily with efficiencies between 80 and 100%. Also, some transfections reagents may work better on certain cells than others, while some reagents can be toxic to specific cell types. For this reason, we recommend to spend some time on optimisation of the transfection conditions for a particular cell type with a reporter construct such as GFP.